Screening of 20 Pantanal Wetland Plants for Anti-Candida Activity using HPLC-DAD-MS/MS and Bioautography to Characterize Active Compounds-Reference-Cited by-同舟云学术

Screening of 20 Pantanal Wetland Plants for Anti-Candida Activity using HPLC-DAD-MS/MS and Bioautography to Characterize Active Compounds

Published:2021-07-14 Issue:03 Volume:8 Page:e96-e103
ISSN:2509-9264
Container-title:Planta Medica International Open
language:en
Short-container-title:Planta Medica International Open

Author:

de Brito Daniela Z.¹,Cassemiro Nadla S.²,de Souza Jeana M. E.²,Damasceno-Junior Geraldo A.³,Oliveira Rodrigo J.⁴,Carollo Carlos A.²^ORCID,Chang Marilene R.¹

Affiliation:

1. Laboratory of Microbiological Research, Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande, Brazil

2. Laboratory of Natural Products and Mass Spectrometry - LaPNEM, Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande, Brazil

3. Laboratory of Botany, Institute of Biosciences, Federal University of Mato Grosso do Sul, Campo Grande, Brazil

4. Center for Studies and Stem Cells, Cell Therapy and Toxicological Genetics – CeTroGen, Maria Aparecida Pedrossian University Hospital, Federal University of Mato Grosso do Sul, Campo Grande, Brazil

Abstract

AbstractThe Pantanal wetland harbors a rich flora with uncharted pharmacological potential. This study evaluated 20 Brazilian Pantanal plants against Candida albicans, C. parapsilosis, C. tropicalis, and C. krusei. Fungal susceptibility was determined by agar diffusion and broth microdilution; active compounds were identified by bioautography and HPLC-DAD-MS/MS. Sesbania virgata, Polygala molluginifolia, and Cantinoa mutabilis extracts and their chloroform and ethyl acetate (EtOAc) fractions exhibited the best activity against all Candida species tested. The EtOAc fraction of P. molluginifolia proved to be more efficient in inhibiting C. parapsilosis and C. krusei growth (Minimum inhibitory concentration of 125 and 62.5 μg/mL, respectively). Bioautography of this fraction revealed two active bands, characterized by HPLC-DAD-MS/MS as a mixture of podophyllotoxin derivatives blumenol, besides some flavonoids. This work demonstrated antifungal potential of P. molluginifolia podophyllotoxin derivatives and the versatility of bioautography with HPLC-DAD-MS/MS to identify the bioactive compounds.

Publisher

Georg Thieme Verlag KG

Subject

General Medicine

Link

http://www.thieme-connect.de/products/ejournals/pdf/10.1055/a-1494-1117.pdf

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