Affiliation:
1. Radiology, The First Affiliated Hospital of Yangtze
University, Jingzhou, China
2. Urology, The First Affiliated Hospital of Yangtze
University, Jingzhou, China
Abstract
AbstractThis study attempted to build a prostate cancer (PC) prognostic risk model with
mitochondrial feature genes. PC-related MTGs were screened for Cox regression
analyses, followed by establishing a prognostic model. Model validity was
analyzed via survival analysis and receiver operating characteristic (ROC)
curves, and model accuracy was validated in the GEO dataset. Combining risk
score with clinical factors, the independence of the risk score was verified by
using Cox analysis, followed by generating a nomogram. The Gleason score,
microsatellite instability (MSI), immune microenvironment, and tumor mutation
burden were analyzed in two risk groups. Finally, the prognostic feature genes
were verified through a q-PCR test. Ten PC-associated MTGs were screened, and a
prognostic model was built. Survival analysis and ROC curves illustrated that
the model was a good predictor for the risk of PC. Cox regression analysis
revealed that risk score acted as an independent prognostic factor. The Gleason
score and MSI in the high-risk group were substantially higher than in the
low-risk group. Levels of ESTIMATE Score, Immune Score, Stromal Score, immune
cells, immune function, immune checkpoint, and immunopheno score of partial
immune checkpoints in the high-risk group were significantly lower than in the
low-risk group. Genes with the highest mutation frequencies in the two groups
were SPOP, TTN, and TP53. The q-PCR results of the feature genes were consistent
with the gene expression results in the database. The 10-gene model based on
MTGs could accurately predict the prognosis of PC patients and their responses
to immunotherapy.