Ultrasonic Vibration For Boosting Fibrinolytic Effect Of Urokinase

Abstract

Boosting effect of ultrasonic vibration on fibrinolytic activity of urokinase has been investigated. Fibrinolysis was studied grouply in (1) experimental fibrin clot with urokinase, (2) clot with urokinase preceded exposure of clot to ultrasonic waves, (3) clot exposed to ultrasonic waves and (4) clot with saline solution. Scanning electro microscopic examinations were made on the clots in each four series. Fibrinolysis was not evident in the clots (3) and (4), though fine cracks were seen in the clots (3). The four hour rate of fibrinolysis was 50% in the clots (1) where as 100% in clots (2).Procedures (1)(2)(3) and (4) were also emplyed in the Chandler’s Loop Technique clot of four hour rotation. The rate of fibrinolysis was 50% in the clot (1) when 60 IU of the urokinase was used, where as 86% in the clot (2) , which scanning electro microscopically gave a feature of decreased fiber structure. Fibrinolysis was accere- rated by ultrasonic vibration in the clot with certain amount of urokinase added, breaking up of the clot being reached in shorter time than the series without ultrasonic wave application.Ultrasonic vibration mechanically might cause microstream in the clot architecture, which could help urokinase contact directly with the fiber net. Scanning electro microscopic examinations revealed morphological representation of enhanced fibrinolysis by ultrasonic vibration. It was suggested that physical ultrasonic vibration could be applied to boost a chemical fibrinolytic effect of urokinase.