Isolation and Characterization of Human Blood Platelet mRNA and Construction of a cDNA Library in λgt11

Author:

Wicki Andreas N1,Walz Alfred1,Gerber-Huber Susan N2,Wenger Roland H1,Vornhagen Rolf3,Clemetson Kenneth J1

Affiliation:

1. Theodor Kocher Institute, Bern, Switzerland

2. Institut für Biochemie und Molekularbiologie, Bern, Switzerland

3. Biotest Serum GmbH, Offenbach, FRG

Abstract

SummaryWe have developed a purification method for the isolation of platelet-specific poly (A+) RNA and demonstrated that human blood platelets, despite the absence of a nucleus, contain stable mRNA. The poly (A+) RNA was used to construct a platelet- specific cDNA expression library in λgtll. The platelet derivation of the purified mRNA was confirmed by identification of membrane glycoprotein Ib (GPIb) message by immunoprecipitation of rabbit reticulocyte lysate translation products with poly- and monoclonal antibodies against GPIbα and by sequencing of a GPIbα cDNA clone

Publisher

Georg Thieme Verlag KG

Subject

Hematology

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