Production of Cryoprecipitate of Intermediate Purity in a Closed System Thaw-Siphon Process

Author:

Mason Ernest C1,Pepper Duncan S2,Griffin Brenda2

Affiliation:

1. The Red Cross Blood Transfusion Service, Brisbane, Queensland, Australia

2. The Scottish National Blood Transfusion Service, Headquarters Unit Laboratory, Edinburgh, Scotland

Abstract

SummaryThe thaw-siphon procedure for routine production of cryoprecipitate is described briefly, and also its extension to a reproducible procedure for production of cryoprecipitates of intermediate purity. For the latter, the average yield of factor VIII is 60% of that in the prefrozon plasma and approximately 30% is present in the supernatant plasma. The product contains 0.53 units of VIII:C per mg of total protein (of which about 70% is clottable). The mean value of the ratio VIII:C/VIII:RAG is about 0.7. The yield and purity of the product compare favourably with those reported for intermediate purity factor VIII concentrates produced by large-scale plasma fractionation laboratories. The procedure offers an alternative pathway to the production of factor VIII concentrate of intermediate purity and can be operated in any blood bank with a minimum of equipment.

Publisher

Georg Thieme Verlag KG

Subject

Hematology

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