The Effect of Phosphorus32, Dextran, Reserpine and Stypturon on Thrombus Formation in Rats

Abstract

SummaryThe effect of the radioisotope P32, hydrolysed dextran, reserpine, stypturon on thrombus formation was determined by injecting these substances into rats before a standardized operation on the jugular vein. 5 hours after the operation 65% of control animals showed a thrombus. 10 days after intravenous injection of 2, 3, or 4 µC/g, the incidence of thrombosis was lowered in direct relation with the reduced platelet counts varying from 65% incidence for counts above 200 000/mm3 to 0% incidence for counts less than 38 000/mm3. Animals injected with dextran, 2 hours, or reserpine, 48 hours before the operation had an incidence of thrombosis of 48% (P <0.01). The incidence of thrombosis was not significantly lowered by mecholyl, producing a hypotension similar to that after dextran, nor by stypturon. The importance of the method for the quantitative evaluation of substances having an effect on thrombus formation is emphasized.