Luminographic Detection of von Willebrand Factor Multimers in Agarose Gels and on Nitrocellulose Membranes-Reference-Cited by-同舟云学术

Luminographic Detection of von Willebrand Factor Multimers in Agarose Gels and on Nitrocellulose Membranes

Published:1990 Issue:02 Volume:63 Page:312-315
ISSN:0340-6245
Container-title:Thrombosis and Haemostasis
language:en
Short-container-title:Thromb Haemost

Author:

Budde U¹,Schneppenheim R²,Plendl H³,Dent J⁴,Ruggeri Z M⁴,Zimmerman T S⁴

Affiliation:

1. The Blood Transfusion Service AK Harburg, Hamburg, FRG

2. The Dept. of Pediatrics, University Clinic, Kiel, FRG

3. The Dept. of Human Genetics, University Clinic, Kiel, FRG

4. The Scripps Clinic and Research Foundation, La Jolla, CA, USA

Abstract

SummaryTwo methods for visualization of vWf multimers were compared with respect to sensitivity and detection of normal vWf and vWd variants IIA, IIB, IIC, IID, HE, and HE Autoradiography and luminography after electrotransfer of vWf multimers onto nitrocellulose showed comparable sensitivity with vWf: Ag detectable after 1:500 dilution of normal plasma. The least sensitive method was luminography in agarose gels with vWf: Ag detectable after 1:300dilution of normal plasma. No difference existed in the banding patterns of plasmas from patients with variant vWd.

Publisher

Georg Thieme Verlag KG

Subject

Hematology

Link

http://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-0038-1645215.pdf

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