Kinetics of the Reaction Between Urokinase and an Inhibitor of Fibrinolysis from Placental Tissue

Author:

Christensen Ulla12,Holmberg L12,Bladh B12,Åstedt B12

Affiliation:

1. The Chemical Laboratory IV, University of Copenhagen, Copenhagen, Denmark, Department of Pediatrics, Allmänna Sjukhuset, Malmö, Sweden

2. The Department of Gynecology and Obstetrics, University of Lund, Lund, Sweden

Abstract

SummaryThe interaction of urokinase (EC 3.4.21.31) and a protein proteinase inhibitor of fibrinolysis partially purified from placental tissue, which inhibits urokinase but not plasmin, was investigated. The preparations of the inhibitor contained no other known proteinase inhibitors. It was found that a 1:1 complex is formed and that the reaction proceeds as a second order, one step process, the association rate constant of which is 4.5 106 M−1 S−1 at pH 8.4, 37° C and 3.0 106 M−1 S−1 at pH 7.3, 37° C. The binding is very tight, the dissociation constant of the inhibitor-urokinase complex was estimated to be Ki≦10−11 M.

Publisher

Georg Thieme Verlag KG

Subject

Hematology

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