Habutobin Splits the Arg16-Gly17 Bond in the Aa Chain of Rabbit Fibrinogen-Reference-Cited by-同舟云学术

Habutobin Splits the Arg16-Gly17 Bond in the Aa Chain of Rabbit Fibrinogen

Published:1997 Issue:06 Volume:77 Page:1127-1128
ISSN:0340-6245
Container-title:Thrombosis and Haemostasis
language:en
Short-container-title:Thromb Haemost

Author:

Kinjoh Kiyohiko¹,Kosugi Tadayoshi¹²,Nakamura Mariko¹,Hanashiro Kazuhiko¹,Sunagawa Masanori¹,Tokeshi Yoshihiro¹,Eguchi Yukinori³

Affiliation:

1. The 1st Department of Physiology, University of the Ryukyus, Okinawa, Japan

2. The Research Center of Comprehensive Medicine, University of the Ryukyus, Okinawa, Japan

3. The Research Laboratory Center, School of Medicine, University of the Ryukyus, Okinawa, Japan

Abstract

SummaryWe reported previously that habutobin, a thrombin-like enzyme from Trimeresurus flavoviridis venom, clotted only rabbit fibrinogen, whereas human, monkey, bovine, dog, rat and guinea-pig fibrinogens were unaffected. In the present study, we investigated the cleavage site of the rabbit Aα chain by habutobin. The fibrinopeptide released by habutobin was identical to the fibrinopeptide A released by thrombin, and its amino acid sequence corresponded to Aα 1-16 of rabbit fibrinogen. It was clarified therefore that habutobin cleaves the Arg16-Gly17 bond in the Aa chain of rabbit fibrinogen.

Publisher

Georg Thieme Verlag KG

Subject

Hematology

Link

http://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-0038-1656124.pdf

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