Platelet Ca2+ Homeostasis: Na+-Ca2+ Exchange in Plasma Membrane Vesicles-Reference-Cited by-同舟云学术

Platelet Ca2+ Homeostasis: Na+-Ca2+ Exchange in Plasma Membrane Vesicles

Published:1987 Issue:03 Volume:57 Page:337-340
ISSN:0340-6245
Container-title:Thrombosis and Haemostasis
language:en
Short-container-title:Thromb Haemost

Author:

Rengasamy Appavoo¹,Soura Soudabeh¹,Feinberg Harold¹

Affiliation:

1. The Department of Pharmacology University of Illinois College of Medicine at Chicago, Chicago, IL, USA

Abstract

SummaryA vesicular plasma membrane-enriched fraction obtained from human platelets exhibited 45Ca2+ uptake in exchange for intravesicular Na+. The rate of Ca2+ uptake was linear up to 4 sec. The apparent Km for Ca2+ was 22 pM and the Vmax 280 pmol/mg/ sec. Ca2+ efflux from Ca2+ loaded vesicles was obtained upon dilution into a NaCl but not a KC1 medium. The extent of Ca2+uptake was monotonically increased as the pH increased from 6 to 9. Na+-Ca2+ exchange was shown to be electrogenic. Ca2+ uptake was distinguished from binding by the induction of Ca2+ release after A23187 addition. These findings support a role for Na+-Ca2+ exchange in human platelet Ca2+ transport.

Publisher

Georg Thieme Verlag KG

Subject

Hematology

Link

http://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-0038-1651129.pdf

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