Affiliation:
1. State Key Laboratory of Fine Chemicals, Frontiers Science Center for Smart Materials Oriented Chemical Engineering, Dalian University of Technology
2. Central Laboratory, Liaoning Cancer Hospital & Institute, Cancer Hospital of Dalian University of Technology
Abstract
AbstractRapidly capturing slight changes in cell surface pH is extremely important to evaluate the rapid diffusion of acidic metabolites into the extracellular environment caused by disease and physiological pH fluctuations of cells. In this work, we designed a membrane-targeted pH probe, Mem-COC18
, based on a novel heterocyclic xanthene-analogous backbone. Mem-COC18
shows specific and stable staining ability towards membrane. Importantly, the fluorescence lifetime of Mem-COC18
is highly sensitive against acidity within membrane, which is in favor of quantifying pH through fluorescence lifetime imaging. Using Mem-COC18
, we recorded pH changes of 0.61 units on the surface of human cervical cancer cells (Hela) during glycolysis. Further on, we observed a robust pH-regulating mechanism of the plasma membrane that the pH fluctuation range within membrane (5.32–6.85) is much smaller than the change in extracellular environment (4.00–8.00). Consequently, we demonstrate a pH probe for quantifying small pH fluctuations within cell membrane that merits further evaluation for biology applications.
Funder
National Natural Science Foundation of China
Dalian Science and Technology Innovation Fund
Fundamental Research Funds for the Central Universities
Cited by
1 articles.
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