Development of the Urinary Tract in Fetal Rats: A Micro-CT Study

Author:

Wille Stephanie12,Peukert Nicole1,Haak Rainer3,Riedel Jan1,Mayer Steffi1,Kluth Dietrich1,Lacher Martin1,Gosemann Jan-Hendrik1,Markel Moritz1

Affiliation:

1. Department of Pediatric Surgery, University of Leipzig, Leipzig, Germany

2. Department of Interdisciplinary Medical Intensive Care, University of Leipzig, Leipzig, Germany

3. Department of Cariology, Endodontology, and Periodontology, University of Leipzig, Leipzig, Germany

Abstract

Abstract Introduction Micro-computed tomography (micro-CT) is an established tool to study fetal development in rodents. This study aimed to use micro-CT imaging to visualize the development of the urinary tract in fetal rats. Materials and Methods Fetal rats from embryonic day (ED) 15, ED17, ED19, ED21, and N0 (newborn) (n = 6 per group; 3 males) were fixed and desiccated using the “critical point” technique. We utilized the micro-CT system (SkyScan) and analyzed the resulting scans with CTAn, DataViewer, and ImageJ to visualize the morphology and quantify the volumes of kidney, bladder, adrenal gland, as well as length of the ureter. Results High-resolution micro-CT showed continuous growth of both kidneys from ED15 to N0, with the highest increase between ED19 and ED21. The length of the ureter increased from ED15 to ED21 and remained stable until birth. The volume of the bladder steadily increased from ED15 to N0.In females, a statistically higher volume of the adrenal gland on ED21 was observed, whereas no sex-specific differences were seen for kidney, ureter, and bladder development. Conclusion Micro-CT depicts an excellent tool to study urinary tract development in the fetal and neonatal rat. It enables the metric quantification of longitudinal anatomic changes in high definition without previous destructive tissue preparation. The present study revealed sex-specific differences of the adrenal gland development and provides comprehensive data for the understanding of fetal urinary tract development, inspiring future research on congenital urological malformations.

Publisher

Georg Thieme Verlag KG

Subject

Surgery,Pediatrics, Perinatology and Child Health

Reference31 articles.

1. Obstructive uropathy;A Becker;Early Hum Dev,2006

2. Congenital obstructive uropathy: its origin and contribution to end-stage renal disease in children;A S Woolf;Adv Ren Replace Ther,2001

3. Congenital urinary bladder outlet obstruction;M K Farrugia;Fetal Matern Med Rev,2010

4. Outcome of urological abnormalities prenatally diagnosed by ultrasound;G Podevin;Fetal Diagn Ther,1996

5. Human intrauterine renal growth expressed in absolute number of glomeruli assessed by the disector method and Cavalieri principle;S A Hinchliffe;Lab Invest,1991

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3