Affiliation:
1. Student Research Committee, School of Medicine, Ardabil University of
Medical Sciences, Ardabil, Iran
2. Research Laboratory for Embryology and Stem Cells, Department of
Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences,
Ardabil, Iran
Abstract
Abstract
Background & Objective 5-fluorouracil (5-FU) is approved for the
treatment of gastric carcinoma (GC), but chemo-resistance limits the application
of it for GC. Thus, the combination of 5-FU with adjuvants such as allicin may
overcome multidrug resistance (MDR).
Methods The anticancer effects of allicin, 5-FU, and allicin/5-FU
on the 5-FU resistant MKN-45 cells were evaluated by MTT assay and DAPi
staining. The expression of the P-glycoprotein (P-gp) and CD44 protein were
determined using immunocytochemistry. We also quantified mRNA expression levels
of WNT5A, Dickkopf-1 (DKK1), and MDR1 in the GC cells.
Results Here, we found that the combination of allicin with 5-FU
significantly increased apoptosis compared to 5-FU alone
(P<0.05). We showed that WNT5A, MDR1, and
DKK1 mRNA expression levels were down-regulated in the allicin- and
allicin/5-FU-treated cells. Indeed, the combination of allicin and 5-FU
significantly decreased the expression of the P-gp and CD44 proteins
(P<0.05).
Conclusion Our findings indicate that the combination of allicin with 5-FU
could reverse multidrug resistance in the GC cells by reducing the expression of
WNT5A, DKK1, MDR1, P-gp, and CD44 levels.
Subject
Drug Discovery,General Medicine
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