Affiliation:
1. Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China
2. Griffith Institute for Drug Discovery, Griffith University, Brisbane, Australia
3. School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China
Abstract
AbstractFour new furostanol saponins (1 – 4) and a new pregane-type saponin (5) along with six known steroidal saponins (6 – 11) were isolated from the rhizomes
of Smilax china. The structures of 1 – 5 were elucidated by extensive analysis of NMR and HR-ESI-MS data in addition to enzymatic hydrolysis and other chemical methods.
Compounds 1, 4, and 11 showed inhibitory activity against the expression of proinflammatory mediators, inducible nitric oxide synthase, interleukin-1β, interleukin-6,
and tumor necrosis factor-α in lipopolysaccharide-induced RAW264.7 cells. Compound 1, at a concentration of 20 µM, decreased the production of inducible nitric oxide synthase,
interleukin-1β, interleukin-6, and tumor necrosis factor-α by 36, 62, 72, and 67%, respectively, which is comparable to that of the positive control dexamethasone.
Funder
Guangdong Basic and Applied Basic Research Foundation
Subject
Organic Chemistry,Complementary and alternative medicine,Drug Discovery,Pharmaceutical Science,Pharmacology,Molecular Medicine,Analytical Chemistry