Large-scale in vitro production of red blood cells from human peripheral blood mononuclear cells-Reference-Cited by-同舟云学术

Large-scale in vitro production of red blood cells from human peripheral blood mononuclear cells

Published:2019-11-04 Issue:21 Volume:3 Page:3337-3350
ISSN:2473-9529
Container-title:Blood Advances
language:en
Short-container-title:

Author:

Heshusius Steven¹²,Heideveld Esther¹,Burger Patrick¹³,Thiel-Valkhof Marijke³,Sellink Erica³,Varga Eszter¹,Ovchynnikova Elina¹,Visser Anna¹,Martens Joost H. A.⁴^ORCID,von Lindern Marieke¹^ORCID,van den Akker Emile¹

Affiliation:

1. Sanquin Research, Department of Hematopoiesis and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands;

2. Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands;

3. Laboratory for Cell Therapy, Sanquin Research, Amsterdam, The Netherlands; and

4. Department of Molecular Biology, Faculty of Science, Nijmegen Centre for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands

Abstract

Key Points This article provides a defined GMP-grade medium and erythroid culture protocol, resulting in >90% enucleated RBC. This article provides a high-resolution database of RNA expression dynamics at daily intervals during terminal erythroid differentiation.

Publisher

American Society of Hematology

Subject

Hematology

Link

http://ashpublications.org/bloodadvances/article-pdf/3/21/3337/1504789/advancesadv2019000689.pdf

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