Diagnostic assay to assist clinical decisions for unclassified severe combined immune deficiency

Author:

Bifsha Panojot1ORCID,Leiding Jennifer W.2,Pai Sung-Yun34ORCID,Colamartino Aurelien B. L.15,Hartog Nicholas67ORCID,Church Joseph A.8,Oshrine Benjamin R.9,Puck Jennifer M.1011,Markert M. Louise1213,Haddad Elie1514ORCID

Affiliation:

1. CHU Sainte-Justine Research Center, Montreal, QC, Canada;

2. Division of Allergy and Immunology, Department of Pediatrics, University of South Florida at Johns Hopkins–All Children’s Hospital, St. Petersburg, FL;

3. Division of Hematology-Oncology, Boston Children’s Hospital, Boston, MA;

4. Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA;

5. Department of Microbiology, Infectious Diseases and Immunology, University of Montreal, Montreal, QC, Canada;

6. Department of Allergy and Immunology, Spectrum Health/Helen DeVos Children’s Hospital, Grand Rapids, MI;

7. Michigan State College of Human Medicine, Grand Rapids, MI;

8. Clinical Immunology and Allergy, Children’s Hospital Los Angeles and Department of Pediatrics, Keck School of Medicine of the University of Southern California, Los Angeles, CA;

9. Cancer and Blood Disorders Institute, Johns Hopkins–All Children’s Hospital, St. Petersburg, FL;

10. Division of Allergy, Immunology and Blood and Marrow Transplantation, Department of Pediatrics, University of California, San Francisco, San Francisco, CA;

11. UCSF Benioff Children’s Hospital, San Francisco, CA;

12. Department of Pediatrics and

13. Department of Immunology, Duke University Medical Center, Durham, NC; and

14. Department of Pediatrics, University of Montreal, Montreal, QC, Canada

Abstract

Key Points 3D organoid T-cell differentiation from a few hundred peripheral blood CD34+ cells was successfully achieved. 3D organoid T-cell differentiation could help physicians distinguish intrinsic from extrinsic defects underlying a clinical SCID phenotype.

Publisher

American Society of Hematology

Subject

Hematology

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