Transcriptome analysis offers a comprehensive illustration of the genetic background of pediatric acute myeloid leukemia

Author:

Shiba Norio123,Yoshida Kenichi4,Hara Yusuke235,Yamato Genki235ORCID,Shiraishi Yuichi6,Matsuo Hidemasa7,Okuno Yusuke8ORCID,Chiba Kenichi6,Tanaka Hiroko9ORCID,Kaburagi Taeko25,Takeuchi Masanobu1,Ohki Kentaro10,Sanada Masashi3,Okubo Jun211,Tomizawa Daisuke12ORCID,Taki Tomohiko13,Shimada Akira14,Sotomatsu Manabu2,Horibe Keizo3ORCID,Taga Takashi15,Adachi Souichi7,Tawa Akio16ORCID,Miyano Satoru17,Ogawa Seishi41819ORCID,Hayashi Yasuhide220

Affiliation:

1. Department of Pediatrics, Yokohama City University Hospital, Yokohama, Japan;

2. Department of Hematology/Oncology, Gunma Children’s Medical Center, Shibukawa, Japan;

3. Clinical Research Center, National Hospital Organization Nagoya Medical Center, Nagoya, Japan;

4. Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan;

5. Department of Pediatrics, Gunma University Graduate School of Medicine, Maebashi, Japan;

6. Center for Cancer Genomics and Advanced Therapeutics, National Cancer Center, Tokyo, Japan;

7. Department of Human Health Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan;

8. Center for Advanced Medicine and Clinical Research, Nagoya University Hospital, Nagoya, Japan;

9. Laboratory of Sequence Analysis, Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan;

10. Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Tokyo, Japan;

11. Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Japan;

12. Division of Leukemia and Lymphoma, Children’s Cancer Center, National Center for Child Health and Development, Tokyo, Japan;

13. Department of Medical Technology, Kyorin University Faculty of Health Sciences, Mitaka, Japan;

14. Department of Pediatrics, Okayama University Graduate School of Medicine, Okayama, Japan;

15. Department of Pediatrics, Shiga University of Medical Science, Ohtsu, Japan;

16. Department of Pediatrics, National Hospital Organization Osaka National Hospital, Osaka, Japan;

17. Laboratory of DNA Information, Human Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, Japan;

18. Institute for the Advanced Study of Human Biology (WPI-ASHBi), Kyoto University, Kyoto, Japan;

19. Department of Medicine, Centre for Haematology and Regenerative Medicine, Karolinska Institute, Stockholm, Sweden; and

20. Institute of Physiology and Medicine, Jobu University, Takasaki, Japan

Abstract

Key Points Using RNA-seq in pediatric AML patients, 5 gene rearrangements were newly identified, including NPM1 and RUNX1 gene rearrangements. RNA-seq unmasked the complexity of gene alterations in pediatric AML by identifying disease-causing alterations in nearly all patients.

Publisher

American Society of Hematology

Subject

Hematology

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