PREPARATION OF STABILIZED SOLUTIONS OF HEMOGLOBIN

Abstract

Abstract It is possible to prepare self-stabilizing solutions of hemoglobin from human erythrocytes by the use of dextrose, nicotinic acid amide and ammonia during the preparation and in the final solutions themselves. Co++, Mn++ and Mg++ ions, nile blue and hexose diphosphate contribute to the speed of stabilization of these solutions. Stabilization is obtained by the faculty of the solutions, presumably by enzymic action, to convert the hemoglobin to the reduced form and to maintain it in this form. The hemoglobin solutions described are suitable for intravenous administration.