Brief Report: Rapid Differentiation of Polypeptide Chains of Hemoglobin by Cellulose Acetate Electrophoresis of Hemolylsates-Reference-Cited by-同舟云学术

Brief Report: Rapid Differentiation of Polypeptide Chains of Hemoglobin by Cellulose Acetate Electrophoresis of Hemolylsates

Published:1969-08-01 Issue:2 Volume:34 Page:230-235
ISSN:0006-4971
Container-title:Blood
language:en
Short-container-title:

Author:

UEDA SATOSHI¹,SCHNEIDER ROSE G.¹²

Affiliation:

1. Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas; present address: Yamuguchi University School of Medicine, Department of Clinical Pathology, Yamuguchi Prefecture, Ube, Japan.

2. Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas.

Abstract

Abstract Separation of the polypeptide chains of hemoglobin and identification of an abnormal chain may be achieved in a few hours by electrophoresis on cellulose acetate in barbital buffer pH 8.6, with 6 M urea. The method is applicable to whole hemolysates to which a large amount of 2-mercaptoethanol has been added. It requires minimal preparative work.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

Link

http://ashpublications.org/blood/article-pdf/34/2/230/575097/230.pdf

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