Affiliation:
1. From the Institut für Hygiene, Leopold-Franzens-Universität Innsbruck, Innsbruck, Austria.
Abstract
Abstract
Multinucleated giant cells (MGC) are a common feature of granulomas that develop during various inflammatory reactions. MGC originate from fusion of monocytes or macrophages, but the exact mechanism of their generation is still unclear. In the present study, we investigated the influence of monocyte to macrophage maturation on the ability of human monocytes/macrophages to fuse with each other. MGC were generated in vitro by stimulation of human peripheral blood monocytes with cytokine containing supernatants. With freshly isolated monocytes, fusion rates of up to 90% were obtained. When monocyte to macrophage maturation was induced by culturing the cells in human serum, fusion rates gradually decreased with advancing time of the preceding culture (corresponding to the stage of differentiation) and almost no MGC formation could be obtained with 8-day-old macrophages. In contrast, fusion rates did not decrease when monocytes had been cultured under serum free conditions before stimulation. When freshly isolated monocytes were added to 1-week cultured macrophages, which had been membrane-labeled with a fluorochrome, fusion between the two populations could be induced. Because the ability for intracellular killing of certain pathogens is reduced in macrophages, fusion with monocytes (newly arriving at the site of inflammation) may represent an attempt to restore this capacity.
Publisher
American Society of Hematology
Subject
Cell Biology,Hematology,Immunology,Biochemistry
Reference51 articles.
1. The granulomatous inflammatory response. A review.;Adams;Am J Pathol,1976
2. Multinucleate giant cells.;Chambers;J Pathol,1978
3. Ueber Riesenzellen mit wandständigen Kernen in Tuberkeln und die fibröse Form des Tuberkels.;Langhans;Virchows Arch Pathol Anat,1868
4. Formation of multinucleated giant cells from human monocyte precursors. Mediation by a soluble protein from antigen- and mitogen-stimulated lymphocytes.;Postlethwaite;J Exp Med,1982
5. Multinucleated giant cells generated in vitro. Terminally differentiated macrophages with down-regulated c-fms expression.;Kreipe;Am J Pathol,1988
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