Elevated soluble MUC1 levels and decreased anti-MUC1 antibody levels in patients with multiple myeloma

Author:

Treon Steven P.1,Maimonis Peter1,Bua Deborah1,Young Gloria1,Raje Noopur1,Mollick Joseph1,Chauhan Dharminder1,Tai Yu-Tzu1,Hideshima Teru1,Shima Yoshihito1,Hilgers Jo1,von Mensdorff-Pouilly Silvia1,Belch Andrew R.1,Pilarski Linda M.1,Anderson Kenneth C.1

Affiliation:

1. From the Department of Adult Oncology, Dana Farber Cancer Institute; and Department of Medicine, Harvard Medical School, Boston, MA; Bayer Diagnostics, Walpole, MA; Academic Hospital, Vrije Universiteit, Amsterdam, The Netherlands; and Department of Medical Oncology, Cross Cancer Institute, Edmonton, Alberta, Canada.

Abstract

Soluble MUC1 (sMUC1) levels are elevated in many MUC1+cancers. We and others have shown that MUC1 is expressed on multiple myeloma (MM) plasma cells and B cells. In this study, we measured sMUC1 levels in bone marrow (BM) plasma from 71 MM patients and 21 healthy donors (HDs), and in peripheral blood (PB) plasma from 42 MM patients and 13 HDs using an immunoassay that detects the CA27.29 epitope of MUC1. sMUC1 levels were found to be significantly greater (mean 31.76 U/mL, range 5.69 to 142.48 U/mL) in MM patient BM plasma versus HD BM plasma (mean 9.68 U/mL, range 0.65 to 39.83 U/mL) (P < .001). Importantly, BM plasma sMUC1 levels were related to tumor burden because sMUC1 levels were significantly higher for MM patients with active disease (34.62 U/mL, range 5.69 to 142.48 U/mL) versus MM patients with minimal residual disease (16.16 U/mL, range 5.7 to 56.68 U/mL) (P = .0026). sMUC1 levels were also elevated in the PB plasma of MM patients (32.79 U/mL, range 4.15 to 148.84 U/mL) versus HDs (18.47 U/mL, range 8.84 to 42.49) (P = .0052). Lastly, circulating immunglobulin M (IgM) and IgG antibodies to MUC1 were measured in 114 MM patients and 31 HDs, because natural antibodies to MUC1 have been detected in patients with other MUC1-bearing malignancies. These studies demonstrated lower levels of circulating IgM (P < .001) and IgG (P = .078) antibodies to MUC1 in MM patients compared with HDs. Our data therefore show that in MM patients, sMUC1 levels are elevated and correlate with disease burden, whereas anti-MUC1 antibody levels are decreased.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

Reference51 articles.

1. The epithelial mucin, Muc1, of milk, mammary gland and other tissues.;Patton;Biochim Biophys Acta.,1995

2. Heterogeneity in production, secretion, and glycosylation of Muc1 epithelial mucin by primary cultures of ovarian carcinoma.;McGuckin;Int J Cancer.,1995

3. Development and characterization of breast cancer reactive monoclonal antibodies directed to the core protein of the human milk mucin.;Burchell;Cancer Res.,1987

4. The breast tumour-associated epithelial mucins and the peanut lectin binding urinary mucins are coded by a single highly polymorphic gene locus ‘PUM.’;Griffiths;Dis Markers.,1988

5. The product of the human Muc1 gene when secreted by mouse cells transfected with the full length cDNA lacks the cytoplasmic tail.;Boshell;Biochem Biophys Res Comm.,1992

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