Lipase Activity in Leukocytes and Macrophages-Reference-Cited by-同舟云学术

Lipase Activity in Leukocytes and Macrophages

Published:1964-11-01 Issue:5 Volume:24 Page:607-615
ISSN:0006-4971
Container-title:Blood
language:en
Short-container-title:

Author:

BRAUNSTEINER H.¹²,DIENSTL F.¹²,SAILER S.¹²,SANDHOFER F.¹²

Affiliation:

1. Department of Medicine, University of Innsbruck, Austria.

2. Department of Internal Medicine, University of Innsbruck, Austria

Abstract

Abstract A method is described for the quantitative determination of alkaline lipase activity of blood cells. The pH optimum of the enzyme in macrophages and lymphocytes from patients with chronic lymph-leukemia is 9.2, the optimum substrate (triglyceride) concentration is 7 x 10-2 M, and the Michaelis constant approximately 4.3 x 10-2 M. Macrophages from guinea pigs exhibit a lipase activity approximately 10 times that of granulocytes and lymphocytes. In granulocytosis an acid lipase could also be demonstrated. The physiologic importance of this finding is discussed.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

Link

http://ashpublications.org/blood/article-pdf/24/5/607/572074/607.pdf

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