Validation of ZAP-70 methylation and its relative significance in predicting outcome in chronic lymphocytic leukemia

Author:

Claus Rainer12,Lucas David M.3,Ruppert Amy S.3,Williams Katie E.3,Weng Daniel4,Patterson Kara5,Zucknick Manuela6,Oakes Christopher C.2,Rassenti Laura Z.7,Greaves Andrew W.7,Geyer Susan3,Wierda William G.8,Brown Jennifer R.9,Gribben John G.10,Barrientos Jacqueline C.11,Rai Kanti R.11,Kay Neil E.12,Kipps Thomas J.7,Shields Peter4,Zhao Weiqiang5,Grever Michael R.3,Plass Christoph2,Byrd John C.3

Affiliation:

1. Division of Hematology, Oncology, and Stem Cell Transplantation, University of Freiburg Medical Center, Freiburg, Germany;

2. Division of Epigenomics and Cancer Risk Factors, German Cancer Research Center, Heidelberg, Germany;

3. Division of Hematology and

4. Division of Medical Oncology, Department of Internal Medicine and the Comprehensive Cancer Center, and

5. Division of Hematopathology, Department of Pathology and the Comprehensive Cancer Center, The Ohio State University, Columbus, OH;

6. Division of Biostatistics, German Cancer Research Center, Heidelberg, Germany;

7. Division of Hematology-Oncology and Central Office of CLL Research Consortium, Moores Cancer Center, University of California San Diego, La Jolla, CA;

8. Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX;

9. Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA;

10. Institute of Cancer, Centre for Medical Oncology, Barts and The London School of Medicine, University of London, London, United Kingdom;

11. Division of Hematology-Oncology, North Shore-Long Island Jewish Health System, New Hyde Park, NY; and

12. Division of Hematology, Mayo Clinic, Rochester, MN

Abstract

Key Points Methylation analysis at ZAP-70 CpG+223 in CLL provides superior prognostic information vs IGHV status or CD38 or ZAP-70 expression. A pyrosequencing method for the feasible assessment of CpG+223 methylation in CLL samples is provided.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

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