RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion-Reference-Cited by-同舟云学术

RNAi screen identifies MAPK14 as a druggable suppressor of human hematopoietic stem cell expansion

Published:2012-06-28 Issue:26 Volume:119 Page:6255-6258
ISSN:0006-4971
Container-title:Blood
language:en
Short-container-title:

Author:

Baudet Aurélie¹,Karlsson Christine¹,Safaee Talkhoncheh Mehrnaz¹,Galeev Roman¹,Magnusson Mattias¹,Larsson Jonas¹

Affiliation:

1. Division of Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, Lund, Sweden

Abstract

We report on a forward RNAi screen in primary human hematopoietic stem and progenitor cells, using pooled lentiviral shRNA libraries deconvoluted by next generation sequencing. We identify MAPK14/p38α as a modulator of ex vivo stem cell proliferation and show that pharmacologic inhibition of p38 dramatically enhances the stem cell activity of cultured umbilical cord blood derived hematopoietic cells. p38 inhibitors should thus be considered in strategies aiming at expanding stem cells for clinical benefit.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

Link

http://ashpublications.org/blood/article-pdf/119/26/6255/1498453/zh802612006255.pdf

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