Standardized flow cytometry for highly sensitive MRD measurements in B-cell acute lymphoblastic leukemia

Author:

Theunissen Prisca1,Mejstrikova Ester2,Sedek Lukasz3,van der Sluijs-Gelling Alita J.4,Gaipa Giuseppe5,Bartels Marius6,Sobral da Costa Elaine7,Kotrová Michaela2,Novakova Michaela26,Sonneveld Edwin4,Buracchi Chiara5,Bonaccorso Paola5,Oliveira Elen7,te Marvelde Jeroen G.1,Szczepanski Tomasz3,Lhermitte Ludovic8,Hrusak Ondrej2,Lecrevisse Quentin9,Grigore Georgiana Emilia10,Froňková Eva2,Trka Jan2,Brüggemann Monika6,Orfao Alberto9,van Dongen Jacques J. M.1,van der Velden Vincent H. J.1

Affiliation:

1. Department of Immunology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands;

2. Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, University Hospital Motol, Prague, Czech Republic;

3. Department of Pediatric Hematology and Oncology, Zabrze, Medical University of Silesia, Katowice, Poland;

4. Dutch Childhood Oncology Group, The Hague, The Netherlands;

5. Centro Ricerca Tettamanti, Clinica Pediatrica Università di Milano Bicocca, Monza, Italy;

6. Department of Hematology, University of Schleswig-Holstein, Campus Kiel, Kiel, Germany;

7. Department of Pediatrics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil;

8. Department of Hematology, Hôpital Necker-Enfants-Malades and Unité Mixte de Recherche Centre National de la Recherche Scientifique 8147, University of Paris Descartes, Paris, France;

9. Cancer Research Center (Instituto de Biología Molecular y Celular del Cancer–Consejo Superior de Investigaciones Científicas), Department of Medicine and Cytometry Service, University of Salamanca, Institute of Biomedical Research of Salamanca, Salamanca, Spain; and

10. Cytognos SL, Salamanca, Spain

Abstract

Key Points Standardized flow cytometry allows highly sensitive MRD measurements in virtually all BCP-ALL patients. If sufficient cells are measured (>4 million), flow cytometric MRD analysis is at least as sensitive as current PCR-based MRD methods.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

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