Microtubule sliding drives proplatelet elongation and is dependent on cytoplasmic dynein

Author:

Bender Markus1ORCID,Thon Jonathan N.23,Ehrlicher Allen J.45,Wu Stephen2,Mazutis Linas346,Deschmann Emoke78,Sola-Visner Martha7,Italiano Joseph E.239,Hartwig John H.1

Affiliation:

1. Division of Translational Medicine and

2. Hematology Division, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA;

3. Platelet BioGenesis, Chestnut Hill, MA;

4. School of Engineering and Applied Sciences, Harvard University, Cambridge, MA;

5. Department of Bioengineering, McGill University, Montreal, QC, Canada;

6. Institute of Biotechnology, Vilnius University, Vilnius, Lithuania

7. Division of Newborn Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA;

8. Division of Neonatology, Department of Women’s and Children’s Health, Karolinska Institutet, Stockholm, Sweden;

9. Department of Surgery, Vascular Biology Program, Boston Children's Hospital, Boston, MA; and

Abstract

Key Points Dynein-dependent microtubule sliding drives proplatelet elongation under static and physiological shear stress conditions. Proplatelet formation is a process that can be divided into repetitive phases: extension, pause, and retraction.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

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