Conventional Western blotting techniques will not reliably quantify p210BCR-ABL1 levels in CML mononuclear cells

Author:

Patel Hetal,Marley Stephen B.,Gordon Myrtle Y.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

Reference5 articles.

1. Copland M, Hamilton A, Elrick LJ, et al. Dasatinib (BMS-354825) targets an earlier progenitor population than imatinib in primary CML but does not eliminate the quiescent fraction. Blood2006; 107:4532–4539.

2. Maxwell SA, Kurzrock R, Parsons SJ, et al. Analysis of p210BCR-ABL tyrosine protein kinase activity in various subtypes of Philadelphia chromosome-positive cells from chronic myelogenous leukaemia patients. Cancer Res1987; 47:1731–1739.

3. Patel H, Marley SB, Gordon MY. Detection in primary chronic myeloid leukaemia cells of p210BCR-ABL1 in complexes with adaptor proteins CBL, CRKL and GRB2. Genes Chromosomes Cancer2006; 45:1121–1129.

4. Schleuning M, Mittermuller J, Kolb HJ. Bcr-abl protein detection in peripheral blood mononuclear cells for follow-up of chronic myelogenous leukaemia patients. Eur J Haematol1999; 62:149–154.

5. Guo JQ, Wang JYJ, Arlinghaus RB. Detection of BCR-ABL proteins in blood cells of benign phase chronic myelogenous leukaemia patients. Cancer Res1991; 51:3048–3051.

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