Affiliation:
1. B-Cell Molecular Immunology Section, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD
Abstract
Abstract
B lymphocyte recirculation through lymph nodes (LNs) requires crossing endothelial barriers and chemoattractant-triggered cell migration. Here we show how LN anatomy and chemoattractant receptor signaling organize B lymphocyte LN trafficking. Blood-borne B cells predominately used CCR7 signaling to adhere to high endothelial venules (HEVs). New B cell emigrants slowly transited the HEV perivenule space, and thereafter localized nearby, avoiding the follicle. Eventually, the newly arrived B cells entered the basal portion of the follicle gradually populating it. In contrast, newly arriving activated B cells rapidly crossed HEVs and migrated toward the lymph node follicle. During their LN residency, recirculating B cells reacquired their sphingosine-1 phospate receptor 1 (S1P1) receptors and markedly attenuated their sensitivity to chemokines. Eventually, the B cells exited the LN follicle by entering the cortical lymphatics or returning to the paracortical cords. Upon entering the lymph, the B cells lost their polarity, down-regulated their S1P1 receptors, and subsequently strongly up-regulated their sensitivity to chemokines. These results are summarized in a model of homeostatic trafficking of B cells through LNs.
Publisher
American Society of Hematology
Subject
Cell Biology,Hematology,Immunology,Biochemistry
Reference42 articles.
1. Lymphocyte homing and homeostasis.;Butcher;Science,1996
2. Ligands for L-selectin: homing, inflammation, and beyond.;Rosen;Annu Rev Immunol,2004
3. Lymphocyte crawling and transendothelial migration require chemokine triggering of high-affinity LFA-1 integrin.;Shulman;Immunity,2009
4. Cords, channels, corridors and conduits: critical architectural elements facilitating cell interactions in the lymph node cortex.;Gretz;Immunol Rev,1997
5. Normal structure, function, and histology of lymph nodes.;Willard-Mack;Toxicol Pathol,2006
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