Isolation and functional characterization of human erythroblasts at distinct stages: implications for understanding of normal and disordered erythropoiesis in vivo

Author:

Hu Jingping12,Liu Jing34,Xue Fumin1,Halverson Gregory5,Reid Marion5,Guo Anqi3,Chen Lixiang3,Raza Azra6,Galili Naomi6,Jaffray Julie7,Lane Joseph8,Chasis Joel Anne3,Taylor Naomi9,Mohandas Narla3,An Xiuli110

Affiliation:

1. Laboratory of Membrane Biology, New York Blood Center, New York, NY;

2. Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha, Hunan, China;

3. Red Cell Physiology Laboratory, New York Blood Center, New York, NY;

4. Molecular Biology Research Center, School of Biological Science and Technology and State Key Laboratory of Medical Genetics of China, Central South University, Changsha, Hunan, China;

5. The Laboratory of Immunochemistry, New York Blood Center, New York, NY;

6. Myelodysplastic Syndromes Center, Columbia University Medical Center, New York, NY;

7. Pediatric Oncology, Memorial Sloan-Kettering Cancer Center, New York, NY;

8. Department of Orthopaedic Surgery, The New York Presbyterian Cornell Hospital, New York, NY;

9. Institut de Génétique Moléculaire de Montpellier, Centre National de la Recherche Scientifique, Montpellier, France; and

10. Department of Bioengineering, Zhengzhou University, Zhengzhou, China

Abstract

Key PointsPurification of staged human erythroblasts should facilitate a comprehensive cellular and molecular characterization of these cell populations. Quantification of human terminal erythropoiesis in vivo provides a powerful means for studying impaired erythropoiesis in human diseases.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

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