The Detroit Strains of Human Epithelial-like Cells from Nonleukemic Peripheral Blood

Author:

BERMAN LAWRENCE1,STULBERG CYRIL S.1

Affiliation:

1. Department of Pathology, Wayne State University College of Medicine, and The Child Research Center of Michigan, Detroit.

Abstract

Abstract 1. The development of 6 strains of Ep-L cells from human blood was reported. In each instance the Ep-L cells appeared in cultures which had some connection with the administration of estrogenic materials, either to the donor of the blood or to the primary explant of blood in vitro. The possible role of estrogens was discussed, but no final conclusions were reached. 2. The development of stable human Ep-L cells did not require the participation of cells from leukemic blood. 3. Morphologic, karyotypic, and mitotic data on the Detroit blood cell strains were presented. 4. The morphogenesis of the Ep-L cells was described. The most likely source of the Ep-L cells in blood cultures appeared to be lymphocytes, but the role of monocytes could not be ruled out. 5. The significance of our findings with respect to transplantation studies was indicated.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

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1. THE ROLE OF THE HOST MESENCHYME IN THE DEVELOPMENT OF TUMOURS AFTER TRANSPLANTATION;Acta Pathologica Microbiologica Scandinavica;2009-08-18

2. IN VITRO INVESTIGATIONS OF THE VIRUS-INDUCED FOWL ERYTHROLEUKEMIA;Acta Pathologica Microbiologica Scandinavica;2009-08-15

3. STUDIES ON THE PHYTOHEMAGGLUTININ OF PHASEOLUS VULGARIS AND ITS MITOGENICITY*;Annals of the New York Academy of Sciences;2006-12-16

4. CULTIVATION OF HEMATOPOIETIC CELLS;Growth, Nutrition, and Metabolism of Cells in Culture;1972

5. Human Peripheral Blood Leucocyte Cultures;Human Chromosome Methodology;1965

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