Hemoglobin Zürich. II. Physicochemical Properties of the Abnormal Hemoglobin-Reference-Cited by-同舟云学术

Hemoglobin Zürich. II. Physicochemical Properties of the Abnormal Hemoglobin

Published:1962-09-01 Issue:3 Volume:20 Page:272-286
ISSN:0006-4971
Container-title:Blood
language:en
Short-container-title:

Author:

BACHMANN FEDOR¹²,MARTI HANS R.¹³

Affiliation:

1. Department of Internal Medicine, University of Zürich, and the Medical Out-Patient Service, University of Basle, Switzerland.

2. Department of Internal Medicine, University of Zürich, Switzerland. At present: Trainee (NIH), Department of Internal Medicine, Washington University, St. Louis, Mo.

3. Medical Out-patient Service, University of Basle, Switzerland.

Abstract

Abstract The physicochemical properties of Hgb Zürich were investigated. This new hemoglobin is characterized by an electrophoretic mobility between Hgb A and Hgb S at pH 8.6. Similarly it runs between Hgb A and Hgb S on amberlite IRC 50 and on carboxymethylcellulose chromatography. Fingerprints of the tryptic digests revealed three unusual peptides; their existence may be explained by the replacement of one histidyl group by an arginyl group in peptides 20, 21 of the β-chain. At 70 C. Hgb Zürich is destroyed three times faster than Hgb A. The erythrocytes and hemolysates of Hgb Zürich carriers show an increased tendency to form methemoglobin. When incubated with acetylphenylhydrazine, the erythrocytes behave like glucose-6-phosphate dehydrogenase-deficient red cells. Carriers of Hgb Zürich tend to develop severe hemolysis after the ingestion of primaquine and a variety of sulfonamides. Glucose-6-phosphate dehydrogenase activity, glutathione stability and methemoglobin reduction tests have been found normal.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

Link

http://ashpublications.org/blood/article-pdf/20/3/272/570698/272.pdf

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