Disc Electrophoresis Study of Serum Proteins from Patients with Multiple Myeloma and Macroglobulinemia-Reference-Cited by-同舟云学术

Disc Electrophoresis Study of Serum Proteins from Patients with Multiple Myeloma and Macroglobulinemia

Published:1963-08-01 Issue:2 Volume:22 Page:152-164
ISSN:0006-4971
Container-title:Blood
language:en
Short-container-title:

Author:

ZINGALE SALVADOR B.¹²,MATTIOLI CARLOS A.¹³,BOHNER HANS D.¹⁴,BUENO MARIA P.¹⁵

Affiliation:

1. Institute for Medical Research, University of Buenos Aires, Medical School, Argentine Republic.

2. National Council for Technical and Scientific Investigations of Argentina, Head, Immunology and Protein Unit, Institute for Medical Research, University of Buenos Aires, Argentina.

3. University of Buenos Aires, Argentina.

4. School of Biochemistry, University, of Buenos Aires, Argentina.

5. Biochemistry Department, Institute of Medical Research, University of Buenos Aires, Argentina.

Abstract

Abstract Sera from 31 patients with plasma cell myeloma and seven with macroglobulinemia were analyzed by the disc electrophoresis method (Ornstein and David). Of the 31 myeloma cases, 24 were found to have a single component. In the remaining cases, in spite of apparent homogeneity in paper electrophoresis, several abnormal components were seen. Ultracentrifugal studies revealed that the heterogeneous myeloma components are a group of globulins with a sedimentation rate between 7S and 19S. Some of the latter components could be easily dissociated by sulfhydryl liberating compounds. Comparison of the results with those obtained by paper and immunoelectrophoresis demonstrated that all the heterogeneous proteins were fast globulins of the β2A type. The possible significance of the heterogeneous myloma proteins is discussed.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

Link

http://ashpublications.org/blood/article-pdf/22/2/152/571180/152.pdf

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