Studies on the A, B, O(H) Blood Groups on Human Cells in Culture-Reference-Cited by-同舟云学术

Studies on the A, B, O(H) Blood Groups on Human Cells in Culture

Published:1965-06-01 Issue:6 Volume:25 Page:944-953
ISSN:0006-4971
Container-title:Blood
language:en
Short-container-title:

Author:

CHESSIN LAWRENCE N.¹²,BRAMSON SHARON¹³,KUHNS WILLIAM J.¹⁴,HIRSCHHORN KURT¹⁵

Affiliation:

1. Departments of Medicine and Pathology, New York University School of Medicine, and the III and IV (N. Y. U.) Medical Divisions, Bellevue Hospital, New York, N. Y.

2. Department of Medicine, New York University School of Medicine, the III and IV (N. Y. U.) Medical Divisions, Bellevue Hospital, New York, N. Y.

3. Department of Pathology, New York University School of Medicine, New York, N. Y.

4. New York University and Bellevue Hospitals, Associate Professor of Pathology, Department of Pathology, New York University School of Medicine, New York, N. Y.

5. Department of Medicine, New York University School of Medicine, the III and W (N. Y. U.) Medical Divisions, Bellevue Hospital, New York, N. Y.

Abstract

Abstract HeLa cells were used in the mixed agglutination reaction to determine optimal conditions for demonstrating blood group H activity by this method. The following parameters were studied in the mixed agglutination reaction: (1) derivation of cell line, (2) cell viability, (3) effects of antibody titer, (4) source and type of antibody. Studies with primary human amnion cells indicated that over a 30-day period of cultivation in vitro there were losses in specific ABO blood group activity. Addition of blood group precursors to establish human amnion cell lines FL-J and F-D indicated that blood group B antigen could be synthesized and maintained in vitro.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

Link

http://ashpublications.org/blood/article-pdf/25/6/944/572430/944.pdf

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