Hemoglobin Biosynthesis in Murine Virus-induced Leukemic Cells In Vitro: Structure and Amounts of Globin Chains Produced-Reference-Cited by-同舟云学术

Hemoglobin Biosynthesis in Murine Virus-induced Leukemic Cells In Vitro: Structure and Amounts of Globin Chains Produced

Published:1972-12-01 Issue:6 Volume:40 Page:823-835
ISSN:0006-4971
Container-title:Blood
language:en
Short-container-title:

Author:

Boyer Samuel H.¹²,Wuu Kuang Dong¹³,Noyes Andrea N.¹⁴,Young Rondall¹⁵,Scher William¹⁶,Friend Charlotte¹⁷,Preisler Harvey D.¹⁸,Bank Arthur¹⁹

Affiliation:

1. Department of Medicine, Division of Medical Genetics and the Clayton Laboratories, The Johns Hopkins University School of Medicine and Hospital, Baltimore, Md.; the Mollie B. Roth Laboratory and Department of Medicine, Center for Experimental Cell Biology, The Mt. Sinai School of Medicine of the City University of New York, New York, N.Y.; and the Department of Medicine, Columbia University Colle

2. Division of Medical Genetics and the Clayton Laboratories, The Johns Hopkins University School of Medicine and Hospital, Baltimore, Md. Recipient of Career Development Award K3-GM- 6308-09.

3. Department of Medicine, Division of Medical Genetics and the Clayton Laboratories, The Johns Hopkins University School of Medicine and Hospital, Baltimore, Md.

4. Division of Medical Genetics and the Clayton Laboratories, The Johns Hopkins University School of Medicine and Hospital, Baltimore, Md.

5. The Johns Hopkins University School of Medicine, Baltimore, Md.

6. Mollie B. Roth Laboratory and Department of Medicine, Center for Experimental Cell Biology,The Mt. Sinai School of Medicine of the City University of New York, New York, N.Y.

7. Mollie B. Roth Laboratory and Department of Medicine, Center for Experimental Cell Biology, The Mt. Sinai School of Medicine of the City University of New York, New York, N.Y. Recipient of National Cancer Institute Grant Ca 10,000.

8. Roth Laboratory and Department of Medicine, Center for Experimental Cell Biology, The Mt. Sinai School of Medicine of the City University of New York, New York, N.Y.

9. Department of Medicine, Columbia University College of Physicians and Surgeons, New York, N.Y.

Abstract

Abstract Tryptic peptides characteristic of mouse hemoglobin α- and diffuse-type β-chains were isolated from dimethylsulfoxide-treated mouse leukemia virus-infected cell cultures. Quantitative estimates of in vitro hemoglobin synthesis by such cultures were obtained by two different methods: by analysis of radioactive amino acid incorporation, and by single-cell radial immunodiffusion assay. The amounts produced, approximately 4 pg/cell/17 hr, reflect a synthetic capacity that is probably sufficient for 32P labeling of hemoglobin messenger RNAs (mRNA) in quantities commensurate with structural characterization of mRNAs by radioautographic methods.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

Link

http://ashpublications.org/blood/article-pdf/40/6/823/754537/823.pdf

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