Affiliation:
1. From the Department of Cell Biology, Faculty of Biology, Complutense University, Madrid, Spain.
Abstract
Abstract
Two dendritic cell (DC) subsets have been identified in the murine system on the basis of their differential CD8α expression. CD8α+ DCs and CD8α− DCs are considered as lymphoid- and myeloid-derived, respectively, because CD8α+ but not CD8α− splenic DCs were generated from lymphoid CD4low precursors, devoid of myeloid reconstitution potential. Although CD8α− DCs were first described as negative for CD4, our results demonstrate that approximately 70% of them are CD4+. Besides CD4− CD8α− and CD4+CD8α− DCs displayed a similar phenotype and T-cell stimulatory potential in mixed lymphocyte reaction (MLR), although among CD8α− DCs, the CD4+ subset appears to have a higher endocytic capacity. Finally, experiments of DC reconstitution after irradiation in which, in contrast to previous studies, donor-type DCs were analyzed without depleting CD4+ cells, revealed that both CD8α+ DCs and CD8α− DCs were generated after transfer of CD4low precursors. These data suggest that both CD8α+ and CD8α− DCs derive from a common precursor and, hence, do not support the concept of the CD8α+ lymphoid-derived and CD8α−myeloid-derived DC lineages. However, because this hypothesis has to be confirmed at the clonal level, it remains possible that CD8α− DCs arise from a myeloid precursor within the CD4low precursor population or, alternatively, that both CD8α+ and CD8α− DCs derive from an independent nonlymphoid, nonmyeloid DC precursor. In conclusion, although we favor the hypothesis that both CD8α+ and CD8α− DCs derive from a lymphoid-committed precursor, a precise study of the differentiation process of CD8α+ and CD8α− DCs is required to define conclusively their origin.
Publisher
American Society of Hematology
Subject
Cell Biology,Hematology,Immunology,Biochemistry
Reference33 articles.
1. Dendritic cell subtypes in mouse lymphoid organs—cross-correlation of surface markers, changes with incubation, and differences among thymus, spleen, and lymph nodes.;Vremec;J Immunol.,1997
2. Definition of dendritic cell subpopulations present in the spleen, Peyer's patches, lymph nodes and skin of the mouse.;Anjuère;Blood.,1999
3. Thymic dendritic cells and T cells develop simultaneously in the thymus from a common precursor population.;Ardavı́n;Nature.,1993
4. Subclass of dendritic cells regulates the response of naive CD8 T cells by limiting their IL-2 production.;Kronin;J Immunol.,1996
5. A subclass of dendritic cells kills CD4 T cells via Fas/Fas-Ligand-induced apoptosis.;Süss;J Exp Med.,1996
Cited by
80 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献