The Cytochemistry of Acute Leukemia: Observations on Glycogen and Neutral Fat in Bone Marrow Aspirates

Author:

BENNETT JOHN M.12,DUTCHER THOMAS F.12

Affiliation:

1. Hematology Service, Clinical Pathology Department, The Clinical Center N.I.H., U.S.P.H.S. Bethesda, Md.

2. Hematology Service, Clinical Pathology Department, National Institutes of Health, Bethesda, Md.

Abstract

Abstract A cytochemical study of 54 cases of acute leukemia utilizing the periodic acid-Schiff reaction for glycogen and Oil Red O for neutral fat was performed on air dried bone marrow smears. The majority of leukemic lymphoblasts revealed significant amounts of glycogen and neutral fat. "Blast" cells in granulocytic leukemia were devoid of glycogen and of neutral fat. The recommendation is made that both of these histochemical stains be employed to attempt to resolve the problem of specific cellular identification in acute leukemia.

Publisher

American Society of Hematology

Subject

Cell Biology,Hematology,Immunology,Biochemistry

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