Biological Activation of Baker's Yeast and the Possibility of Enrichment of Bakery Products with Peptides

Abstract

Introduction. The stimulation of the fermentation activity of baker's yeast in baking and giving additional nutritional value to mass consumption food products is an important area of ​​scientific research.Purpose. The purpose of the study is to evaluate the effect of partial replacement of a salt solution with an enzymatic hydrolyzate of cow colostrum on the activation of baking yeast Saccharomyces cerevisiae when kneading the dough and to study the possibility of enriching bakery products with biologically active peptides.Materials and Methods. The research materials were model dough samples prepared without yeast activation and on activated yeast using enzymatic hydrolyzate of bovine colostrum. The presence of peptides in the dough samples was determined on a MALDI-TOF mass spectrometer, decryption was performed using the Mascot database, the Peptide Fingerprint option (Matrix Science, USA) using the Protein NCBI database.Results. It has been established that the replacement of the sodium chloride solution used in the dough formulation with the enzymatic hydrolyzate of bovine colostrum has a positive effect on the activation of metabolic processes in the yeast cells of Saccharomyces cerevisiae. The results obtained indicate that, compared with the control, the lifting force of pressed baker's yeast with a complete replacement of the salt solution with the enzymatic hydrolyzate of cow colostrum increased by 26%, for dried yeast, the lifting force increased by 20% compared to the control sample. In model dough samples, a biologically active peptide present in the used enzymatic hydrolyzate of bovine colostrum was identified, the amino acid sequence and molecular weight of the isolated peptide were determined.Conclusions. The use of enzymatic hydrolyzate of bovine colostrum in the technology of bakery products will speed up the production process and enrich the bread with biologically active peptides. But at the same time, it is necessary to conduct additional studies on the effect of activated yeast on the quality and shelf life of biologically active peptides in finished bread.