Abstract
The emergence of drug-resistant strains reason for the search for natural alternatives. The study aimed to evaluate the anthelmintic activity of Nerium oleanderleaf extract against Allolobophora caliginosain vitro. The concentrations (25 mg/mL, 50 mg/mL, and 100 mg/mL), were tested, andthe results were expressed in paralysis time and the death of worms. Albendazole (40 mg/mL), and distilled water were used as standard drug and control. The FT-IR and GC-MS analysis of the extract showed the presence of 12 and 13 compounds responsible foranthelmintic activity. There was a dose-dependent increase in effectiveness over time during 2.5, 5, 7.5, and 10 minutes. The death reached 97% and 100%, respectively when (100 mg/mL) after 10 minutes. Histological examination and scanning electron microscopy showed noticeable abnormalities and a cuticular layer of the body surface had shrunk in all treated worms. The current experiment results show that oleander has powerful anti-Acaliginosa action.