Author:
Romero María C.,Mariazzi Aldo A.
Abstract
Eight bacterial strains were isolated from Chascomús Pond (Buenos Aires Province) and their capacity to growth at low concentrations of glycolate and glucose, as a solé source of organic carbón was evaluated.Substrate affinities (Kt valúes) and other kinetic parameters were measured by Wright & Hobbie methodology with 14C-labelled glycollate and MC-labelled glucose as substrates. Four o f the eight puré culture studied followed Michaelis-Menten uptakekinetics giving good straight lines after the Lineweaver-Burke transfonnation.Vmax valúes o f natural popuiations and the experimental ones were similar for glycollate; for glucose the experimental valúes were a little higher. This suggest the possibility o f applying the experimental Kt valúes to natural popuiations as a way of evaluation o f the natural substrate concentration.
Publisher
Universidad Nacional del Nordeste
Reference25 articles.
1. AKAGI, Y., TAGA, N. & SIMIDU, V. 1977. Isolation and distribution of ligotrophic bacteria. Can J. Microbio!. 23:981-987.
2. AKAGI, Y. & TAGA, N. 1980. Uptake o f D-glucose and L-proline by oligotrophic and heterotrophic maeine bacteria. Can. J. Microbiol. 26:454-459.
3. CONZONNO, V. H. & FERNANDEZ CIRELLI, A. 1985. Soluble humic substances from the affluents o f Chascomús Pond (Argentina), farch. Hydrobiol. 109: 305-314.
4. CONZONNO, V. H. & CLAVERIE, E.- F. 1990. Chemical characteristics of the water of Chascomús Pond (Provincia de Buenos Aires, Argentina). Limnological implications. Rev. Brasil. Biol. 50: 15-21.
5. DANGAUS, N. V. 1976. Descripción sistemática de los parámetros morfológicos considerados en las lagunas pampásicas. Limnobios. 1: 35-39.