Abstract
Only a few azoxy natural products have been identified despite their intriguing biological activities. Azodyrecins D–G, four new analogs of aliphatic azoxides, were identified from two Streptomyces species by a reactivity-based screening that targets azoxy bonds. A biological activity evaluation demonstrated that the double bond in the alkyl side chain is important for the cytotoxicity of azodyrecins. An in vitro assay elucidated the tailoring step of azodyrecin biosynthesis, which is mediated by the S-adenosylmethionine (SAM)-dependent methyltransferase Ady1. This study paves the way for the targeted isolation of aliphatic azoxy natural products through a genome-mining approach and further investigations of their biosynthetic mechanisms.
Funder
Uehara Memorial Foundation
Asahi Glass Foundation
Ministry of Education, Culture, Sports, Science and Technology
Sumitomo Foundation
Japan Science and Technology Agency
Naito Foundation
Daiichi Sankyo Foundation of Life Science
Japan Agency for Medical Research and Development
Cited by
2 articles.
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