Immunoassay Interference by a Commonly Used Blood Collection Tube Additive, the Organosilicone Surfactant Silwet L-720

Author:

Bowen Raffick AR1,Chan Yung1,Ruddel Mark E1,Hortin Glen L1,Csako Gyorgy1,Demosky Stephen J2,Remaley Alan T1

Affiliation:

1. Department of Laboratory Medicine, Warren Grant Magnuson Clinical Center, and

2. Molecular Disease Section, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD

Abstract

Abstract Background: A small number of immunoassays on several different types of analyzers were recently adversely affected by tube additives in Becton Dickinson (BD) Vacutainer® SST™, SST II, and Microtainer™ blood collection tubes. We examined the effect of a commonly used tube surfactant, Silwet™ L-720, on immunoassays and the mechanism for the interference. Methods: Immunoassays were performed on serum supplemented with Silwet L-720 on the IMMULITE™ 2500 and AxSYM™ analyzers. Direct effects of the surfactant on the chemiluminescent detection step of immunoassays and on antibody immobilization on the solid phase were examined. Results: Increasing the final surfactant concentration from 0 to 400 mg/L in serum significantly increased (∼51%) the apparent total triiodothyronine (TT3) concentrations measured on the IMMULITE 2500 but not the AxSYM analyzer. Several other competitive, but not noncompetitive, assays were also significantly affected by the surfactant on the IMMULITE 2500 analyzer. The effect was independent of serum components, and the surfactant had no direct effect on chemiluminescence reactions. The capture antibody, however, was displaced from the solid phase by incubation with solutions containing surfactant under conditions similar to the IMMULITE TT3 assay. Conclusions: The Silwet L-720 surfactant, which is used to coat the inner surfaces of tubes, appears to account for previously reported immunoassay interference by BD Vacutainer SST blood collection tubes. One of the mechanisms for the interference is the desorption of antibodies from the solid phase by the surfactant. The results identify an important factor in the selection of suitable blood collection tube surfactants and provide an approach for solving similar tube-assay interference problems in the future.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

Reference35 articles.

1. Cohen R, Dastane AN, inventors. Additive preparation and method of use thereof. US Patent 5,340,166;2003..

2. Anraku H, Shoji Y, inventors. Vacuum blood collection tubes. US Patent 8,565,334;1989..

3. Kessler SB, inventor. Apparatus for the separation of blood components. US Patent 2,578,864;1981..

4. Sampson M, Ruddel M, Albright S, Elin RJ. Positive interference in lithium determinations from clot activator in collection container. Clin Chem1997;43:675-679.

5. Landt M, Wilhite TR, Smith CH. A new plastic evacuated tube with plasma separator. J Clin Lab Anal1995;9:101-106.

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