Affiliation:
1. Research-Development Institute for Plant Protection Bucharest, Romania
Abstract
"Many techniques for the histological study of the invertebrate gonads topography are described. For
arthropods, a major drawback is the presence of the exoskeleton, which is a hardly permeable structure to the
aqueous reagents with mechanical resistance also. Therefore, use of specific reagents and techniques, adapted for
the study of their internal structures is a must. Several methods have been tested in this study like aqueous
topographical fixation solutions based on picric acid (Bouin), mercury (II) chloride („Susa” Heidenhain) as well
as alcoholic ones, picric acid (Duboscq-Brasil), mercury (II) chloride (Apathy) or acetic acid (Carnoy II) based.
For the i) dehydration methods using absolute ethanol or 2-propanol, and ii) embedding ones the following
methods were tested: paraffin with benzene, tert-butanol or pyridine as intermediary solvent (Gabe), paraffincelloidin double inclusion with diethyl ether: ethanol (1:1) as solvent (Pfuhl). After embedding and casting the
paraffin blocks, these were cut at 5-10μ, the section ribbons were spread on distilled water and glued on slides
with glycerol-albumine Mayer. For the double-embedded material, the sections were glued with Ruyter’s
solution, with or without application of collodion. Heidenhain’s „Azan”, Mann-Dominici, Van Gieson and Gabe
staining methods were used. For permanent slides, natural Canada balsam or artificial one “Caedax” were
dissolved in xylene (mixture of isomers). Several procedures were tested for fixing solution, intermediary
solvent, dehydrating, embedding and staining. The aim of this study was to optimise an efficient method in terms
of results and reproducibility. Here, one of the successful methods is detailed (Heidenhain’s „Susa” fixation,
ethanol dehydrating, paraffin embedding and Heidenhain’s „Azan” staining), to be followed by a series of
optimized methods in future articles. The results obtained are reproducible, allowing the investigation methods
of the structural modification of the white fly gonads induced by the chemical or biological control methods
standardization.
"
Publisher
Institutul de Cercetare Dezvoltare pentru Protectia Plantelor Bucuresti
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