Stepwise unfolding of a multi-tryptophan protein MPT63 with immunoglobulin-like fold: detection of zone-wise perturbation during guanidine hydrochloride-induced unfolding using phosphorescence spectroscopy-Reference-Cited by-同舟云学术

Stepwise unfolding of a multi-tryptophan protein MPT63 with immunoglobulin-like fold: detection of zone-wise perturbation during guanidine hydrochloride-induced unfolding using phosphorescence spectroscopy

Published:2016 Issue:66 Volume:6 Page:61077-61087
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Mukherjee Manini¹²³⁴,Ghosh Ranendu⁵⁶⁷⁴,Chattopadhyay Krishnananda⁵⁶⁷⁴,Ghosh Sanjib¹²³⁴

Affiliation:

1. Department of Chemistry

2. Presidency University

3. Kolkata 700 073

4. India

5. Structural Biology & Bio-Informatics Division

6. Indian Institute of Chemical Biology

7. Kolkata 700 032

Abstract

The present article highlights the simple inexpensive and rapid technique of phosphorescence spectroscopy at 77 K that reveals the environment of all the four tryptophan residues of a protein MPT63 during guanidine hydrochloride induced unfolding.

Funder

Science and Engineering Research Board

Council of Scientific and Industrial Research

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/RA/C6RA06545G

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