Label-free fluorescence detection of kinase activity using a gold nanoparticle based indicator displacement assay
Author:
Affiliation:
1. Department of Chemical Sciences
2. University of Padova
3. 35131 Padova
4. Italy
5. Department of Pharmaceutical and Pharmacological Sciences
6. Padova
Abstract
A straightforward fluorescence indicator-displacement assay (IDA) has been developed for the quantitative analysis of ATP→ADP conversion.
Funder
European Research Council
Publisher
Royal Society of Chemistry (RSC)
Subject
Organic Chemistry,Physical and Theoretical Chemistry,Biochemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2015/OB/C4OB02052A
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1. The Raison D'Être of Constitutively Active Protein Kinases: The Lesson of CK2
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