Magnetic ELISA of aflatoxin B1 – pre-concentration without elution

Author:

Petrakova A. V.1234,Urusov A. E.1234,Zherdev A. V.1234,Dzantiev B. B.1234

Affiliation:

1. Bach Institute of Biochemistry

2. Research Center of Biotechnology of the Russian Academy of Sciences

3. 119071 Moscow

4. Russia

Abstract

This study proposes an ELISA variant based on the application of highly dispersed (average diameter – 10 nm) magnetic iron oxide nanoparticles as carriers for the adsorbable antibodies.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Reference43 articles.

1. The immunoassay handbook: theory and applications of ligand binding, ELISA and related techniques, ed., D. Wild, Elsevier Science, Oxford, 2013

2. Theoretical limitations on immunoassay sensitivity

3. B. B. Dzantiev and A. V.Zherdev, in Portable Biosensing of Food Toxicants and Environmental Pollutants, ed. D. P. V. Nikolelis, Theodoros Erdem, Arzum Nikoleli and Georgia-Paraskevi, Taylor & Francis, London – New York, 2013, pp. 161–196

4. A Decade of Development in Immunoassay Methodology

5. Layer-by-Layer Construction of Novel Biofunctional Fluorescent Microparticles for Immunoassay Applications

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