LNA-enhanced DNA FIT-probes for multicolour RNA imaging-Reference-Cited by-同舟云学术

LNA-enhanced DNA FIT-probes for multicolour RNA imaging

Published:2016 Issue:1 Volume:7 Page:128-135
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Hövelmann F.¹²³⁴⁵,Gaspar I.⁵⁶⁴,Chamiolo J.¹²³⁴,Kasper M.¹²³⁴,Steffen J.¹²³⁴,Ephrussi A.⁵⁶⁴,Seitz O.¹²³⁴

Affiliation:

1. Department of Chemistry

2. Humboldt University Berlin

3. D-12489 Berlin

4. Germany

5. European Molecular Biology Laboratory (EMBL) Heidelberg

6. 69117 Heidelberg

Abstract

A surrogate nucleobase based on 4,4'-cyanine provided up to 195-fold enhancement of red fluorescence upon complementary RNA binding. A mixture of FIT probes allowed the localization of oskar mRNA and other polyadenylated mRNA molecules in developing oocytes from Drosphila melanogaster by wash-free FISH and STED.

Funder

Einstein Stiftung Berlin

Deutsche Forschungsgemeinschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/SC/C5SC03053F

Reference54 articles.

1. Molecular Beacons: Probes that Fluoresce upon Hybridization

2. A Highly Sensitive, Excimer-Controlled Molecular Beacon