Quantification of N-hydroxysuccinimide and N-hydroxysulfosuccinimide by hydrophilic interaction chromatography (HILIC)-Reference-Cited by-同舟云学术

Quantification of N-hydroxysuccinimide and N-hydroxysulfosuccinimide by hydrophilic interaction chromatography (HILIC)

Published:2015 Issue:15 Volume:7 Page:6443-6448
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Klykov Oleg¹²³,Weller Michael G.¹²³

Affiliation:

1. BAM Federal Institute for Materials Research and Testing

2. Division 1.5 Protein Analysis

3. Germany

Abstract

NHS and sulfo-NHS esters are used for many different bioconjugations. A simple and robust chromatographic method is presented, reaching a detection limit of about 1 mg/L of NHS. The purity determination of a commercial fluorescent dye NHS ester and other applications are shown.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/AY/C5AY00042D

Reference24 articles.

1. N-Hydroxysuccinimide Esters in Peptide Synthesis

2. The Use of Esters of N-Hydroxysuccinimide in Peptide Synthesis

3. The use of activated esters in peptide synthesis

4. 002 Determination of triazine herbicides by ELISA ? Optimization of enzyme tracer synthesis

5. Extremely sensitive and selective antibodies against the explosive 2,4,6-trinitrotoluene by rational design of a structurally optimized hapten

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