A generic microfluidic biosensor of G protein-coupled receptor activation – impedance measurements of reversible morphological changes of reverse transfected HEK293 cells on microelectrodes-Reference-Cited by-同舟云学术

A generic microfluidic biosensor of G protein-coupled receptor activation – impedance measurements of reversible morphological changes of reverse transfected HEK293 cells on microelectrodes

Published:2015 Issue:65 Volume:5 Page:52563-52570
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Srivastava Saurabh K.¹²³⁴⁵,Ramaneti Rajesh⁶⁷⁴,Roelse Margriet¹²³⁴,Tong Hien Duy⁶⁷⁴,Vrouwe Elwin X.⁸⁹⁴,Brinkman Aldo G. M.¹⁰¹¹¹²⁴¹³,de Smet Louis C. P. M.¹⁰¹¹¹²⁴¹³,van Rijn Cees J. M.⁵¹⁴¹⁵⁴⁶,Jongsma Maarten A.¹²³⁴¹³

Affiliation:

1. Plant Research International

2. Wageningen UR

3. 6708 PB Wageningen

4. The Netherlands

5. Laboratory of Organic Chemistry

6. Nanosens B.V.

7. 7201 JE Zutphen

8. Micronit Microfluidics B.V.

9. 7521 PV Enschede

10. Department of Chemical Engineering

11. Delft University of Technology

12. 2628 BL Delft

13. NanoNextNL

14. WageningenUR

15. 6703 HB Wageningen

Abstract

Flowcell with micro-IDEs (250–500 μm) covered with both stable and reverse transfected cells overexpressing membrane receptors to demonstrate impedance responses to serial injections of analyte.

Funder

Technologiestichting STW

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/RA/C5RA04976H

Reference45 articles.

1. Label-free whole-cell assays: expanding the scope of GPCR screening

2. Label-Free Cell-Based Assays for GPCR Screening

3. Deorphanizing G Protein-Coupled Receptors by a Calcium Mobilization Assay