A single amino acid Gly-tag enables metal-free protein purification-Reference-Cited by-同舟云学术

A single amino acid Gly-tag enables metal-free protein purification

Published:2020 Issue:48 Volume:11 Page:13137-13142
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Purushottam Landa¹²³⁴,V. B. Unnikrishnan¹²³⁴,Rawale Dattatraya Gautam¹²³⁴,Gujrati Mansi⁵²³⁴,Mishra Surya Dev¹²³⁴⁵,T. K. Sajeev⁵²³⁴,Reddy Neelesh C.¹²³⁴,Adusumalli Srinivasa Rao¹²³⁴,Mishra Ram Kumar⁵²³⁴,Rai Vishal¹²³⁴^ORCID

Affiliation:

1. Department of Chemistry

2. Indian Institute of Science Education and Research Bhopal

3. Bhopal 462 066

4. India

5. Department of Biological Sciences

Abstract

Gly-tag resin precisely captures and releases a protein with one glycine at the N-terminus. The user-friendly protocol delivers analytically pure protein free of metal contaminants.

Funder

Science and Engineering Research Board

Department of Atomic Energy, Government of India

Indian Institute of Science Education and Research Bhopal

Department of Biotechnology, Ministry of Science and Technology, India

Council of Scientific and Industrial Research, India

University Grants Commission

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/SC/D0SC01153C

Reference27 articles.

1. W. Nes , Protein Purification: Principles, High-Resolution Methods, and Applications , ed. J. C. Janson and L. Rydén , John Wiley & Sons , New York , 2nd edn, 1988

2. Protein production and purification

3. Metal chelate affinity chromatography, a new approach to protein fractionation

4. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

5. Overview of Affinity Tags for Protein Purification

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