Mass spectrometry-based monitoring of millisecond protein–ligand binding dynamics using an automated microfluidic platform-Reference-Cited by-同舟云学术

Mass spectrometry-based monitoring of millisecond protein–ligand binding dynamics using an automated microfluidic platform

Published:2016 Issue:9 Volume:16 Page:1544-1548
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Cong Yongzheng¹²³⁴,Katipamula Shanta¹²³⁴,Trader Cameron D.¹²³⁴,Orton Daniel J.⁵²³⁴,Geng Tao¹²³⁴,Baker Erin S.⁵²³⁴,Kelly Ryan T.¹²³⁴

Affiliation:

1. Environmental Molecular Sciences Laboratory

2. Pacific Northwest National Laboratory

3. Richland

4. USA

5. Biological Sciences Division

Abstract

We present a novel microfluidic platform that features rapid mixing of protein and ligand solutions, variable incubation times, and an integrated electrospray ionization source for mass spectrometry-based monitoring of protein–ligand binding dynamics.

Funder

National Institute of Environmental Health Sciences

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2016/LC/C6LC00183A

Reference38 articles.

1. Ion mobility-mass spectrometry of intact protein–ligand complexes for pharmaceutical drug discovery and development

2. Structure-Function of the G Protein–Coupled Receptor Superfamily

3. Drug–protein binding: a critical review of analytical tools